REVERSED PHASE HPLC DETERMINATION OF TOTAL HOMOCYSTEINE, CYSTEINE, CYSTEINYL GLYCINE, GLUTATHIONE IN PLASMA OF EPILEPTIC PATIENTS
نویسندگان
چکیده
The fast and accurate reversed-phase HPLC method was predicated on qualitative quantitative estimation of the total concentrations essential thiols in plasma adults volunteers compared with epileptic patients, which is l-cysteine (Cys) homocysteine (tHys), cysteinyl glycine (Cys-Gly), glutathione (GSH) cysteamine (Cstm), respectively. samples (50) patients were classified into three groups; non-anti-epileptic drug consisted 20 (12 male, eight female) who mainly diagnosed idiopathic generalized epilepsy. mean age 34 ± 13 (range 19–48) years. second group 30 receiving two types anti-epileptic drugs, 18 male six Carbamazepine, 12 (7 males five phenytoin. 32 11 Compared 31 healthy (20 female). controls 9 26–49 years). duration treatment months). All measurement done using pre-column derivatization bromobimane, derivative each standard mixture baseline separated C18-DB (50 x 4.6 mm ID) column, 3μm particle size. regression coefficients for deviation SD within-run ranged from 0.09 to 8.40 μmol/L, between-run 0.15to 9.16 μmol/L; analytical procedure gave good linearity range between 1.25 µmole/ l, detection limit 0.1 µmole /L all thiol groups. Analytical recovery 96.9–107.4 %; concentration cysteine slightly higher than females; it 221± 75 adult 190± 44 μmol/L females, while t-homocysteine (10.55 ±2.45 vs. 9.79 1.88 results observed that value significantly Carbamazepine phenytoin volunteers. Mean values lower, showed no significant difference sex- age-dependent.
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ژورنال
عنوان ژورنال: The American journal of medical sciences and pharmaceutical research
سال: 2023
ISSN: ['2689-1026']
DOI: https://doi.org/10.37547/tajmspr/volume05issue07-08